Ort:
Vienna BioCenter 5 (VBC5)
Hörsaal der Strukturchemie, PART A
1030 Wien
Genome-scale loss-of-function screens are currently the state of the art to do functional characterization in several model systems. The current method of choice for e ectively introducing targeted loss-of-function mutations is the RNA-guided clustered regularly interspaced short palindrome repeats (CRISPR)-associated nuclease Cas9 (Shalem et al., 2014). In this project CRISPR/Cas9 is used to knock out genes, which are suspected to cause microcephaly, in organoids. During this project we developed bioinformatic tools to analyze the screening data. One of the major challenges for the analysis was to identify the di erent sources for the errors in the next-generation-sequencing data. After doing multiple step of hamming distance based error correction we are able to identify genes that are associated with microcephaly. Thus a combination of experimental work and sophisticated bioinformatics led to the success of the project.